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	OpenPCR Basic Data Category: Health URL (first publication): https://openpcr.org/ Project status: Technical documentation Maturity of the project: no no no Other Assembly instructions are editable: Bill of materials is editable: Design files are in original format: No Free redistribution is allowed licence: No Project management Open-o-meter: 0 Product category: Business & Industrial Contains original non-electronic hardware: Contains original electronic hardware: Contains original software:

Description

OpenPCR is a project to develop open source hardware, software, and protocols to perform PCR and Real-Time PCR reactions, and a community dedicated to openness in science and applying the fundamental technologies of PCR to global problems

We aim to make the fundamental technologies of PCR and Real-Time PCR universally accessible, without exorbitant costs or intellectual property issues, and to apply this fundamental technology to challenging global issues, such as point-of-care medical diagnostics in developing countries and ensuring the safety of the worldâ€™s food supplies. We envision a world where molecular diagnostics are routinely used by all and do not require specialized knowledge.

At its core, PCR is a method of detecting the presence of a particular DNA sequence. This is useful in myriad applications, such as determining whether an individual is infected with HIV or Malaria, or whether leafy vegetables are contaminated with E. Coli or Listeria. Unfortunately traditional Real-Time PCR Machines capable of detecting these pathogens typically cost upwards of $30,000 US dollars and are not suitable for field usage.

OpenPCR kits are currently manufactured and distributed by Chai, a company founded by Josh Perfetto and Jessie Ho to democratize access to molecular diagnostics. The OpenPCR may also be fabricated directly by end users from design documents downloadable from this website.

Accessible hardware is only half the battle. In addition to providing low-cost machines, we aim to openly share the knowledge needed to make use of these machines, such as PCR protocols and assays, within the community. We believe you shouldnâ€™t have to be a molecular biologist to put the well understood techniques of PCR to work for you, no matter your application or research.

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Public cad repository for electronic hardware
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Assembly instructions
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-|desc=<div><div><p>Your open-source<br/>PCR Thermocycler</p><p>OpenPCR is a low-cost yet accurate thermocycler<br/>you build yourself, capable of reliably controlling PCR<br/>reactions for DNA detection, sequencing, and limitless<br/>other applications.</p><br/><p>now available</p></div><br/><br/><div><li><div><div><div><p>PCR Reactions<br/>that Work</p><p>Eliminate failed reactions and inconsistent results<br/>caused by poor thermal control on older equipment.<br/>OpenPCR delivers temperatures within +/- 0.5 C of the target</p></div><br/><div><p>A Thermocycler<br/>for Anywhere</p><p>Whether you are a small lab on a budget or looking<br/>to do PCR in the field, the low cost and small size of<br/>OpenPCR makes PCR accessible to anyone.</p></div></div><br/><div><div><p>Anyone can<br/>Build and Use One</p><p>Assembling the kit is easy with IKEA-like build instructions,<br/>while a Windows/OS X GUI makes designing your<br/>PCR protocol a breeze.</p></div><br/><div><p>100%<br/>Open Source</p><p>The complete software and CAD of the OpenPCR are released<br/>as open source. Modify the design to suit your needs,<br/>or simply support the movement to make science more open.</p></div></div></div></li></div><br/><div><div>TECHNICAL SPECIFICATIONS</div><br/><br/><div><li><div><div><div><div>THERMALCYCLING &amp; PCR</div><br/><div>SAMPLE CAPACITY:</div><div>200 uL</div><div>TEMP ACCURACY:</div><div>+/- 0.5 C</div><div>AVERAGE RAMP RATE:</div><div>1 C/s</div><div>TEMP UNIFORMITY:</div><div>+/- 0.3 C</div><div>TEMP RANGE:</div><div>20 - 105 C</div><div>HEATED LID TEMP:</div><div>Ambient - 120 C</div><div>THERMOCYCLING TECHNOLOGY:</div><div>Thermoelectric module</div></div><div><div>CONTROL FEATURES</div><p>Programmable initial, cyclic,<br/>and final steps</p><p>Operates with PC connection and<br/>in disconnected mode</p></div></div><div><div><div>COMMUNICATION</div><div><div>DISPLAY:</div><div>4 line backlit LCD</div><div>PORTS:</div><div>1 USB A</div></div></div><br/><div><div>SOFTWARE</div><div><div>PLATFORM:</div><div>Windows, Mac, Linux</div><div>PROGRAM MEMORY:</div><div>Unlimited on PC, thermocycler stores last program<br/>when disconnected</div><div>LICENSE:</div><div>GPLv3</div></div></div></div><div><div><div>DIMENSIONS &amp; WEIGHT</div><div>HEIGHT:</div><div>25 CM</div><div>WIDTH:</div><div>13 CM</div><div>DEPTH:</div><div>20 CM</div><div>WEIGHT:</div><div>3.5 KG</div></div><div><div>ELECTRICAL</div><div>VOLTAGE:</div><div>100-240 V</div><div>FREQUENCY:</div><div>50-60 Hz</div><div>POWER CONSUMPTION:</div><div>200 W</div></div></div></div></li></div></div><div><div>BUILD YOUR OPEN PCR</div><div><p>Build in Three hours<br/>with three tools</p><p>OpenPCR takes about 3 hours to build. Your kit includes all<br/>the parts for building an OpenPCR. The only tools you’ll need<br/>are a normal philips head screwdriver, a 2 mm flathead<br/>screwdriver, and pliers.</p></div></div><br/><br/><div><div>RUN OPENPCR</div><div><div><p>Pre-run checklist<br/>and software</p><p>Make sure your OpenPCR is ready to rock Run<br/>these diagnostic tests starting on page 69 of the<br/>Instruction manual:</p><br/><div><div>Power Test<br/>Check that OpenPCR boots and is<br/>assembled correctly.</div><br/><div>Run your first test protocol<br/>Verify that thermal control is working.</div><br/><div>Adjust the heated lid<br/>Use an empty PCR tube to set the lid<br/>height adjustment.</div></div></div><div><p>OpenPCR Software<br/>The OpenPCR application runs on both Windows<br/>and Mac OS X platforms using Adobe Air.</p><br/><br/><p>Tell us about your experience<br/>Love your OpenPCR or suggest new features?<br/>Drop us a line at .</p></div></div></div><div><div>OPEN SOURCE DESIGN</div><div><p>100% OPEN SOURCE<br/>FILES AND INSTRUCTIONS.</p><p>All OpenPCR software, design files and instructions are<br/>licensed under the .</p><p>Read more about the .</p></div></div></div>
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 |open_o_meter=5
 |product_category=Business & Industrial
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 |original_elect_h=No
 |original_soft=No
-|cad_files_editable=No
+|desc=OpenPCR is a project to develop open source hardware, software, and protocols to perform PCR and Real-Time PCR reactions, and a community dedicated to openness in science and applying the fundamental technologies of PCR to global problems
-|assembly_instructions_editable=No
-|bill_materials_editable=Yes
+We aim to make the fundamental technologies of PCR and Real-Time PCR universally accessible, without exorbitant costs or intellectual property issues, and to apply this fundamental technology to challenging global issues, such as point-of-care medical diagnostics in developing countries and ensuring the safety of the worldâ€™s food supplies. We envision a world where molecular diagnostics are routinely used by all and do not require specialized knowledge.
-|free_redist_allowed_licence=Yes
+At its core, PCR is a method of detecting the presence of a particular DNA sequence. This is useful in myriad applications, such as determining whether an individual is infected with HIV or Malaria, or whether leafy vegetables are contaminated with E. Coli or Listeria. Unfortunately traditional Real-Time PCR Machines capable of detecting these pathogens typically cost upwards of $30,000 US dollars and are not suitable for field usage.
+OpenPCR kits are currently manufactured and distributed by [https://angel.co/chai Chai], a company founded by [https://www.linkedin.com/in/jperfetto/ Josh Perfetto] and [https://www.linkedin.com/in/jessie-ho/ Jessie Ho] to democratize access to molecular diagnostics. The OpenPCR may also be fabricated directly by end users from design documents downloadable from this website.
+Accessible hardware is only half the battle. In addition to providing low-cost machines, we aim to openly share the knowledge needed to make use of these machines, such as PCR protocols and assays, within the community. We believe you shouldnâ€™t have to be a molecular biologist to put the well understood techniques of PCR to work for you, no matter your application or research.
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