FluoPi

FluoPi Basic Data Category: Microscopes URL (first publication): https://www.biomaker.org/projects/open-source-autonomous-imaging-station Project status: Technical documentation Maturity of the project: no no no Other Assembly instructions are editable: Bill of materials is editable: Design files are in original format: No Free redistribution is allowed licence: No Project management Open-o-meter: 0 Product category: Cameras & Optics Contains original non-electronic hardware: Contains original electronic hardware: Contains original software:

Description

We proposed to create a set of tools for scientific research, engineering and teaching biology at the scale of whole colonies and entire plates. First, we designed and built a standalone tool for imaging and analysing fluorescence in biological samples at three different spectra and at a range of scales from individual colonies to whole plates (easily extensible to plant cells and even whole organisms such as C. elegans and Marchantia spp). The system is self-contained and autonomous, including hardware and software for image capture, programmed sequences (e.g. timelapse), and quantitative analysis of samples. We also developed a simple genetic toolkit for the production of fluorescent and pigmented bacteria complementing the device. We have optimized genetic construction protocols and adopted fluorescent markers that can be used with 470 nm single excitation. Altogether, our system allows for construction and integral experimentation with hardware, software and genetic instructions from off-the-shelve, open access and distributed resources.

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Public cad repository for non-electronic hardware
Public cad repository for electronic hardware
Public code repository
Assembly instructions
Bill of materials
Link github